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Human CXCL4/PF4 ELISA Kit

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EK0726

  • 96T ¥2480
  • 货期: 现货
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产品简介>

产品名称
Human CXCL4/PF4 ELISA Kit
规格/价格
96T|2480
指标别名
C X C motif chemokine 4; CXCL4; Iroplact; Oncostatin A; PF 4; PF4; platelet factor 4; SCYB4
免疫原
E.coli, E32-S101
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名称
PF4
基因名全称
platelet factor 4
Uniprot ID
P02776  
文献引用格式
Human CXCL4/PF4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0726)
背景资料
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0726-CAP96-well plate precoated with anti- human CXCL4 antibody1
EK0726-STLyophilized recombinant human CXCL4 standard10ng/tube×2
EK0726-DABiotinylated anti- human CXCL4 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3551700479833715475118
Standard deviation24.8579.9249.4924.2691.27348.02
CV(%)74.75.27.25.96.8
典型数据示例
Concentration (pg/ml)015631262512502500500010000
O.D.0.0110.0650.1170.1790.330.6051.0471.824
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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