产品名称
Rat CXCL4/PF4 ELISA Kit
指标别名
C X C motif chemokine 4; CXCL4; Iroplact; Oncostatin A; PF 4; PF4; platelet factor 4; SCYB4
免疫原
Expression system for standard: E.coli; Immunogen sequence: V30-S105
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat CXCL4/PF4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1651)
背景资料
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1651-CAP | 96-well plate precoated with anti- rat CXCL4 antibody | 1 |
| EK1651-ST | Lyophilized recombinant rat CXCL4 standard | 10ng/tube×2 |
| EK1651-DA | Biotinylated anti- rat CXCL4 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 205 | 1658 | 5065 | 199 | 1676 | 4964 |
| Standard deviation | 8.81 | 72.95 | 379.87 | 9.35 | 87.15 | 416.97 |
| CV(%) | 4.3% | 4.4% | 7.5% | 4.7% | 5.2% | 8.4% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.01 | 0.049 | 0.072 | 0.12 | 0.226 | 0.442 | 0.923 | 1.874 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)
联系我们
关注我们
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
