Human CD10/MME ELISA Kit

96T/2480

Atriopeptidase; CALLA; CD10; Enkephalinase; EPN; membrane metallo endopeptidase; MME; MME; CD10; NEP; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11

CHO, Y52-W750

There is no detectable cross-reactivity with other relevant proteins.

125 pg/ml - 8000 pg/ml

MME

membrane metallo-endopeptidase

P08473  

Human CD10/MME ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0851)

CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

20-25分钟

Catalog number	Description	Quantity
EK0851-CAP	96-well plate precoated with anti- human CD10 antibody	1
EK0851-ST	Lyophilized recombinant human CD10 standard	10ng/tube×2
EK0851-DA	Biotinylated anti- human CD10 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	201	956	4316	216	881	4302
Standard deviation	14.87	51.62	233.06	17.92	60.78	305.44
CV(%)	7.4	5.4	5.4	8.3	6.9	7.1

Concentration(pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.097	0.148	0.196	0.319	0.462	0.858	1.328	1.965

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h