Mouse CD10/MME ELISA Kit

96T/2480

Atriopeptidase; CALLA; CD10; Enkephalinase; EPN; membrane metallo endopeptidase; MME; MME; CD10; NEP; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11

Expression system for standard: NS0; Immunogen sequence: Y52-W750

There is no detectable cross-reactivity with other relevant proteins.

93.7pg/ml-6000pg/ml

MME

Neprilysin

Q61391  

Mouse CD10/MME ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0852)

Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), CD10, and common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Catalog number	Description	Quantity
EK0852-CAP	96-well plate precoated with anti- mouse CD10 antibody	1
EK0852-ST	Lyophilized recombinant mouse CD10 standard	10ng/tube×2
EK0852-DA	Biotinylated anti- mouse CD10 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	165	1021	3228	175	951	3447
Standard deviation	7.26	41.86	216.27	8.22	55.15	255.07
CV(%)	4.4%	4.1%	6.7%	4.7%	5.8%	7.4%

Concentration(pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.021	0.097	0.179	0.316	0.555	1.122	1.737	2.261

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h