产品名称
Human CEA/CEACAM5 ELISA Kit
指标别名
Carcinoembryonic antigen; CD66e; CEA; CEACAM5; Meconium antigen 100
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
基因名全称
carcinoembryonic antigen-related cell adhesion molecule 5
文献引用格式
Human CEA/CEACAM5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0904)
背景资料
Carcinoembryonic antigen(CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0904-CAP | 96-well plate precoated with anti- human CEA antibody | 1 |
| EK0904-ST | Lyophilized recombinant human CEA standard | 20ng/tube×2 |
| EK0904-DA | Biotinylated anti- human CEA antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 776 | 1668 | 10176 | 724 | 1662 | 10501 |
| Standard deviation | 39.57 | 125.1 | 407.04 | 39.09 | 137.94 | 420.04 |
| CV(%) | 5.1 | 7.5 | 4 | 5.4 | 8.3 | 4 |
典型数据示例
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.041 | 0.089 | 0.132 | 0.281 | 0.432 | 0.87 | 1.334 | 2.05 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)