Mouse CXCL5 ELISA Kit

96T|2480

C X C motif chemokine 5; CXCL5; ENA 78; ENA 78(1 78); ENA78; SCYB5; Small inducible cytokine B5

Expression system for standard: E.coli; Immunogen sequence: V45-A118

There is no detectable cross-reactivity with other relevant proteins.

15.6pg/ml-1000pg/ml

CXCL5

C-X-C motif chemokine 5

P50228  

Mouse CXCL5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0919)

C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Catalog number	Description	Quantity
EK0919-CAP	96-well plate precoated with anti- mouse CXCL5 antibody	1
EK0919-ST	Lyophilized recombinant mouse CXCL5 standard	10ng/tube×2
EK0919-DA	Biotinylated anti- mouse CXCL5 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	35	166	388	36	150	360
Standard deviation	2.17	8.13	18.23	2.48	8.1	20.52
CV(%)	6.2%	4.9%	4.7%	6.9%	5.4%	5.7%

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.006	0.137	0.214	0.375	0.722	1.097	1.734	2.209

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h