指标别名
MPO; myeloperoxidase
免疫原
Expression system for standard: NS0; Immunogen sequence: M16-T718
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse MPO ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0943)
背景资料
Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0943-CAP | 96-well plate precoated with anti- mouse MPO antibody | 1 |
| EK0943-ST | Lyophilized recombinant mouse MPO standard | 10ng/tube×2 |
| EK0943-DA | Biotinylated anti- mouse MPO antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 354 | 1809 | 5328 | 362 | 1718 | 5171 |
| Standard deviation | 19.11 | 115.77 | 266.4 | 24.97 | 137.44 | 346.45 |
| CV(%) | 5.4% | 6.4% | 5% | 6.9% | 8% | 6.7% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.038 | 0.155 | 0.261 | 0.449 | 0.831 | 1.277 | 1.825 | 2.085 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)