产品名称
Human PDGF-BB/PDGFB ELISA Kit
指标别名
platelet derived growth factor subunit B; IBGC5; PDGF-2; PDGF2; SIS; SSV; c-sis; PDGFB
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
platelet-derived growth factor beta polypeptide
文献引用格式
Human PDGF-BB/PDGFB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0956)
背景资料
Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer(PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide(PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0956-CAP | 96-well plate precoated with anti- human PDGF-BB antibody | 1 |
| EK0956-ST | Lyophilized recombinant human PDGF-BB standard | 10ng/tube×2 |
| EK0956-DA | Biotinylated anti- human PDGF-BB antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 94 | 199 | 788 | 88 | 194 | 725 |
| Standard deviation | 4.13 | 12.53 | 39.4 | 5.19 | 12.8 | 36.25 |
| CV(%) | 4.4 | 6.3 | 5 | 5.9 | 6.6 | 5 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.011 | 0.071 | 0.133 | 0.247 | 0.468 | 0.866 | 1.581 | 2.505 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)