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Rat PDGF-BB/PDGFB ELISA Kit

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EK1698

  • 96T ¥2480
  • 货期: 现货
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产品名称
Rat PDGF-BB/PDGFB ELISA Kit
规格/价格
96T|2480
指标别名
transforming growth factor beta 1; CED; DPD1; IBDIMDE; LAP; TGF-beta1; TGFB; TGFbeta; Tgfb1
免疫原
Expression system for standard: E.coli; Immunogen sequence: S82-T190
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5pg/ml-4000pg/ml
基因名称
Pdgfb
基因名全称
transforming growth factor, beta 1
Uniprot ID
Q05028  
文献引用格式
Rat PDGF-BB/PDGFB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1698)
背景资料
PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
显色时间
20-25分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1698-CAP96-well plate precoated with anti- rat PDGF-BB antibody1
EK1698-STLyophilized recombinant rat PDGF-BB standard10ng/tubex2
EK1698-DABiotinylated anti- rat PDGF-BB antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)10865110711217141110
Standard deviation4.4333.8558.97.652.8486.58
CV(%)4.1%5.2%5.5%6.3%7.4%7.8%
典型数据示例
Concentration (pg/ml)062.5125250500100020004000
O.D.0.0390.0930.1450.250.430.7581.3351.846
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

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