产品名称
Rat PDGF-BB/PDGFB ELISA Kit
指标别名
transforming growth factor beta 1; CED; DPD1; IBDIMDE; LAP; TGF-beta1; TGFB; TGFbeta; Tgfb1
免疫原
Expression system for standard: E.coli; Immunogen sequence: S82-T190
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
transforming growth factor, beta 1
文献引用格式
Rat PDGF-BB/PDGFB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1698)
背景资料
PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1698-CAP | 96-well plate precoated with anti- rat PDGF-BB antibody | 1 |
| EK1698-ST | Lyophilized recombinant rat PDGF-BB standard | 10ng/tubex2 |
| EK1698-DA | Biotinylated anti- rat PDGF-BB antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 108 | 651 | 1071 | 121 | 714 | 1110 |
| Standard deviation | 4.43 | 33.85 | 58.9 | 7.6 | 52.84 | 86.58 |
| CV(%) | 4.1% | 5.2% | 5.5% | 6.3% | 7.4% | 7.8% |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.039 | 0.093 | 0.145 | 0.25 | 0.43 | 0.758 | 1.335 | 1.846 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)