产品名称
Human Progranulin/GRN ELISA Kit
指标别名
acrogranin; GEP; GP88; granulin; Granulin A Granulin 5; Granulin B Granulin 4; Granulin C Granulin 6; Granulin D Granulin 7; Granulin E; Granulin F Granulin 3; Granulin G Granulin 2; Granulins; GRN; PCDGF; PEPI; PGRN; Proepithelin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human Progranulin/GRN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0973)
背景资料
Granulin, also known as Proepithelin(PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0973-CAP | 96-well plate precoated with anti- human Progranulin antibody | 1 |
| EK0973-ST0 | Lyophilized recombinant human Progranulin standard | 100ng/tube×2 |
| EK0973-DA | Biotinylated anti- human Progranulin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 507 | 1173 | 9591 | 518 | 1169 | 9230 |
| Standard deviation | 36.35 | 71.9 | 321.3 | 16.37 | 88.96 | 699.63 |
| CV(%) | 7.2 | 6.1 | 3.4 | 3.2 | 7.6 | 7.6 |
典型数据示例
| Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.008 | 0.556 | 0.791 | 1.059 | 1.644 | 1.886 | 2.057 | 2.019 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk
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