产品名称
Human Periostin/POSTN ELISA Kit
指标别名
OSF 2; OSF2; Osteoblast specific factor 2; PDLPOSTN; Periostin; PN; POSTN; RP11 412K4.1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
periostin, osteoblast specific factor
文献引用格式
Human Periostin/POSTN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0985)
背景资料
Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0985-CAP | 96-well plate precoated with anti- human Periostin antibody | 1 |
| EK0985-ST | Lyophilized recombinant human Periostin standard | 10ng/tube×2 |
| EK0985-DA | Biotinylated anti- human Periostin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 188 | 1707 | 4824 | 180 | 1664 | 4867 |
| Standard deviation | 8.64 | 124.61 | 192.96 | 11.34 | 136.44 | 199.54 |
| CV(%) | 4.6 | 7.3 | 4 | 6.3 | 8.2 | 4.1 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.016 | 0.078 | 0.128 | 0.25 | 0.442 | 0.853 | 1.434 | 2.097 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)