产品名称
Mouse Complement C5a ELISA Kit
指标别名
C5; Complement C5; complement component 5; CPAMD4
免疫原
Expression system for standard: E.coli; Immunogen sequence: N679-R755
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Complement C5a ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0987)
背景资料
C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0987-CAP | 96-well plate precoated with anti- mouse Complement C5a antibody | 1 |
| EK0987-ST | Lyophilized recombinant mouse Complement C5a standard | 10ng/tube×2 |
| EK0987-DA | Biotinylated anti- mouse Complement C5a antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 38 | 229 | 499 | 35 | 224 | 532 |
| Standard deviation | 1.78 | 16.94 | 38.92 | 2.1 | 18.81 | 49.47 |
| CV(%) | 4.7% | 7.4% | 7.8% | 6% | 8.4% | 9.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.06 | 0.135 | 0.214 | 0.338 | 0.568 | 0.893 | 1.461 | 1.912 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
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