产品名称
Mouse Complement C5a Quick ELISA Kit
指标别名
C5; Complement C5; complement component 5; CPAMD4
免疫原
Expression system for standard: E.coli; Immunogen sequence: N679-R755
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
complement component 5
文献引用格式
Mouse Complement C5a Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0987)
背景资料
C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
试剂盒内容
| Catalog number | Description | Quantity |
| FEK0663-CAP | 96-well plate precoated with anti-Mouse Complement C5a antibody | 1 |
| FEK0663-ST | lyophilized recombinant Mouse Complement C5a standard | 10ng/tube×2 |
| FEK0663-A | Mouse Complement C5a Antibody A | 3ml |
| FEK0987-B | Mouse Complement C5a Antibody B | 3ml |
| AR1106-1 | Sample Diluent | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 43 | 331 | 501 | 37 | 339 | 575 |
| Standard deviation | 1.80 | 22.50 | 26.14 | 2.77 | 25.42 | 50.60 |
| CV(%) | 4.2% | 6.8% | 7.9% | 6.1% | 7.5% | 8.8% |
典型数据示例
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.025 | 0.181 | 0.302 | 0.551 | 0.986 | 1.669 | 2.168 | 2.310 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)