产品名称
Human PON1 ELISA Kit
指标别名
A esterase 1; Aromatic esterase 1; ESA; K 45; paraoxonase 1; PON; PON 1; PON1; Serum aryldialkylphosphatase 1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
文献引用格式
Human PON1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1141)
背景资料
Serum paraoxonase/arylesterase 1(PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1(paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein(HDL).
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1141-CAP | 96-well plate precoated with anti- human PON1 antibody | 1 |
| EK1141-ST | Lyophilized recombinant human PON1 standard | 2ng/tube×2 |
| EK1141-DA | Biotinylated anti- human PON1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 49 | 203 | 917 | 47 | 199 | 834 |
| Standard deviation | 2.15 | 13.19 | 42.18 | 2.16 | 15.52 | 40.86 |
| CV(%) | 4.4 | 6.5 | 4.6 | 4.6 | 7.8 | 4.9 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.041 | 0.092 | 0.141 | 0.23 | 0.422 | 0.768 | 1.348 | 2.377 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin)