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Mouse WISP1/CCN4 ELISA Kit

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EK1195

  • 96T ¥2480
  • 货期: 现货
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产品名称
Mouse WISP1/CCN4 ELISA Kit
规格/价格
96T|2480
指标别名
CCN family member 4; CCN4; WISP 1; WISP1; WISP-1; WISP1c; WISP1i; WISP1tc; Wnt 1 induced secreted protein
免疫原
Expression system for standard: NS0; Immunogen sequence: T23-N367
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2pg/ml-2000pg/ml
基因名称
CCN4
Uniprot ID
O54775  
文献引用格式
Mouse WISP1/CCN4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1195)
背景资料
WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.
试剂盒内容
Catalog numberDescriptionQuantity
EK1195-CAP96-well plate precoated with anti- mouse WISP1 antibody1
EK1195-STLyophilized recombinant mouse WISP1 standard10ng/tube×2
EK1195-DABiotinylated anti- mouse WISP1 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)6626187869265906
Standard deviation4.2916.760.585.5818.8167.95
CV(%)6.5%6.4%6.9%8.1%7.1%7.5%
典型数据示例
Concentration (pg/ml)031.262.512525050010002000
O.D.0.0260.0970.1640.2750.4620.8541.4922.483
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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