Human WISP1/CCN4 ELISA Kit

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EK1204

96T ￥2480
货期：现货

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产品名称

Human WISP1/CCN4 ELISA Kit

规格/价格

96T|2480

指标别名

CCN family member 4; CCN4; WISP 1; WISP1; WISP-1; WISP1c; WISP1i; WISP1tc; Wnt 1 induced secreted protein

免疫原

NSO, T23-N367

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

31.2 pg/ml - 2000 pg/ml

基因名称

CCN4

基因名全称

WNT1 inducible signaling pathway protein 1

Uniprot ID

O95388

文献引用格式

Human WISP1/CCN4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1204)

背景资料

WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK1204-CAP	96-well plate precoated with anti- human WISP1 antibody	1
EK1204-ST	Lyophilized recombinant human WISP1 standard	10ng/tube×2
EK1204-DA	Biotinylated anti- human WISP1 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	98	231	917	94	225	930
Standard deviation	5.68	10.16	46.76	6.95	11.47	47.43
CV(%)	5.8	4.4	5.1	7.4	5.1	5.1

典型数据示例

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.012	0.035	0.062	0.114	0.252	0.527	1.032	2.041

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human WISP1/CCN4 ELISA Kit

EK1204

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

All ELISA(1) All Species Human(1) 清除

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