产品名称
Human SPARC ELISA Kit
指标别名
Basement membrane protein 40; BM 40; ON; Osteonectin; SPARC
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
0.78 ng/ml - 50 ng/ml
基因名全称
secreted protein, acidic, cysteine-rich (osteonectin)
文献引用格式
Human SPARC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1210)
背景资料
SPARC(secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus(domain I), 2) a cysteine- rich(domain II), 3) a hydrophilic region(domain III) and 4) an EF hand motif at the carboxy terminus region(domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1210-CAP | 96-well plate precoated with anti- human SPARC antibody | 1 |
| EK1210-ST | Lyophilized recombinant human SPARC standard | 50ng/tube×2 |
| EK1210-DA | Biotinylated anti- human SPARC antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1247 | 5311 | 21553 | 1248 | 5203 | 22753 |
| Standard deviation | 93.52 | 393.01 | 1012.99 | 99.84 | 457.86 | 1296.92 |
| CV(%) | 7.5 | 7.4 | 4.7 | 8 | 8.8 | 5.7 |
典型数据示例
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.061 | 0.131 | 0.208 | 0.345 | 0.579 | 0.936 | 1.478 | 2.187 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Human Milk
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