指标别名
F TCF; Hepatocyte growth factor; Hepatopoeitin A; HGF; HGFB; HPTA; Scatter factor; SF
免疫原
Expression system for standard: NS0; Immunogen sequence: Q33-R495 (α) & V496-L728 (β)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Hepatocyte growth factor
文献引用格式
Mouse HGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1217)
背景资料
Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1217-CAP | 96-well plate precoated with anti- mouse HGF antibody | 1 |
| EK1217-ST | Lyophilized recombinant mouse HGF standard | 10ng/tube×2 |
| EK1217-DA | Biotinylated anti- mouse HGF antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 249 | 1468 | 5963 | 259 | 1383 | 5909 |
| Standard deviation | 16.18 | 89.54 | 298.15 | 20.72 | 106.49 | 372.26 |
| CV(%) | 6.5% | 6.1% | 5% | 8% | 7.7% | 6.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.022 | 0.094 | 0.144 | 0.278 | 0.52 | 0.902 | 1.468 | 2.07 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (EDTA, citrate)