Rat HGF ELISA Kit

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EK1301

96T ￥2280
货期：现货

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产品简介>

产品名称

Rat HGF ELISA Kit

规格/价格

96T|2280

指标别名

F TCF; Hepatocyte growth factor; Hepatopoeitin A; HGF; HGFB; HPTA; Scatter factor; SF

免疫原

Expression system for standard: NS0; Immunogen sequence: Q33-R495 (alpha) & V496-L728 (beta)

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

156pg/ml-10000pg/ml

基因名称

HGF

基因名全称

hepatocyte growth factor (hepapoietin A; scatter factor)

Uniprot ID

P17945

文献引用格式

Rat HGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1301)

背景资料

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000.1 The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus.2 HGF may serve as a paracrine mediator to control placental development and growth.3 This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.4 The gene encoding the human HGF is assigned to human chromosome 7.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK1301-CAP	96-well plate precoated with anti- rat HGF antibody	1
EK1301-ST	Lyophilized recombinant rat HGF standard	10ng/tube×2
EK1301-DA	Biotinylated anti- rat HGF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	223	1031	4762	201	1018	4682
Standard deviation	12.48	78.35	371.43	12.46	90.6	412.01
CV(%)	5.6%	7.6%	7.8%	6.2%	8.9%	8.8%

典型数据示例

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.028	0.097	0.189	0.35	0.685	1.166	1.727	2.029

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Rat HGF ELISA Kit

EK1301

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

检验物种

检测方法

样本类型

其他介绍>

引用文献(0)>

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