指标别名
CD6; CD6 molecule; T12; TP120
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human CD6 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1221)
背景资料
CD6(Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich(SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What's more, this gene may be associated with susceptibility to multiple sclerosis.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1221-CAP | 96-well plate precoated with anti- human CD6 antibody | 1 |
| EK1221-ST | Lyophilized recombinant human CD6 standard | 10ng/tube×2 |
| EK1221-DA | Biotinylated anti- human CD6 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 255 | 1450 | 4911 | 250 | 1511 | 5268 |
| Standard deviation | 14.79 | 72.5 | 378.14 | 16.5 | 75.55 | 447.78 |
| CV(%) | 5.8 | 5 | 7.7 | 6.6 | 5 | 8.5 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.041 | 0.133 | 0.207 | 0.347 | 0.559 | 0.942 | 1.417 | 1.848 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)