指标别名
CD6; CD6 molecule; T12; TP120
免疫原
Expression system for standard: NS0; Immunogen sequence: L18-G396
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
T-cell differentiation antigen CD6
文献引用格式
Mouse CD6 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1287)
背景资料
CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What’s more, this gene may be associated with susceptibility to multiple sclerosis.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1287-CAP | 96-well plate precoated with anti- mouse CD6 antibody | 1 |
| EK1287-ST | Lyophilized recombinant mouse CD6 standard | 10ng/tube×2 |
| EK1287-DA | Biotinylated anti- mouse CD6 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 133 | 525 | 1268 | 129 | 485 | 1288 |
| Standard deviation | 5.58 | 35.17 | 92.56 | 6.45 | 37.83 | 114.63 |
| CV(%) | 4.2% | 6.7% | 7.3% | 5% | 7.8% | 8.9% |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.045 | 0.117 | 0.179 | 0.292 | 0.519 | 0.949 | 1.738 | 2.354 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)