Human Prostasin/PRSS8 ELISA Kit

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EK1272

96T ￥2480
货期：现货

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产品名称

Human Prostasin/PRSS8 ELISA Kit

规格/价格

96T|2480

指标别名

CAP1; PROSTASIN; protease; serine; 8; PRSS8; Serine protease 8

免疫原

CHO, A33-G319

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

1.56 ng/ml - 100 ng/ml

基因名称

PRSS8

基因名全称

protease, serine, 8

Uniprot ID

Q16651

文献引用格式

Human Prostasin/PRSS8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1272)

背景资料

Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK1272-CAP	96-well plate precoated with anti- human Prostasin antibody	1
EK1272-ST	Lyophilized recombinant human Prostasin standard	100ng/tube×2
EK1272-DA	Biotinylated anti- human Prostasin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	3254	12396	41368	3115	13251	40618
Standard deviation	247.3	595	1944.29	267.89	689.05	2599.55
CV(%)	7.6	4.8	4.7	8.6	5.2	6.4

典型数据示例

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.035	0.096	0.16	0.251	0.432	0.763	1.289	1.839

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human Prostasin/PRSS8 ELISA Kit

EK1272

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

All ELISA(1) All Species Mouse(1) 清除

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