产品名称
Mouse Prostasin/PRSS8 ELISA Kit
指标别名
CAP1; PROSTASIN; protease; serine; 8; PRSS8; Serine protease 8
免疫原
Expression system for standard: NS0; Immunogen sequence: A30-Q289
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Prostasin/PRSS8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1273)
背景资料
Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1273-CAP | 96-well plate precoated with anti- mouse Prostasin antibody | 1 |
| EK1273-ST | Lyophilized recombinant mouse Prostasin standard | 50ng/tube×2 |
| EK1273-DA | Biotinylated anti- mouse Prostasin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1504 | 6502 | 26206 | 1649 | 6732 | 27351 |
| Standard deviation | 102.27 | 435.63 | 1205.47 | 113.78 | 484.7 | 1723.11 |
| CV(%) | 6.8% | 6.7% | 4.6% | 6.9% | 7.2% | 6.3% |
典型数据示例
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.029 | 0.098 | 0.138 | 0.233 | 0.483 | 0.729 | 1.27 | 2.094 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine