产品名称
Mouse Chemerin/RARRES2 ELISA Kit
指标别名
Chemerin; HP10433; RAR responsive protein TIG2; RARRES2; TIG2
免疫原
Expression system for standard: E.coli; Immunogen sequence: T20-K162
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Retinoic acid receptor responder protein 2
文献引用格式
Mouse Chemerin/RARRES2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1330)
背景资料
Chemerin, also known as RARRES2 or TIG2, is a protein that is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1330-CAP | 96-well plate precoated with anti- mouse Chemerin antibody | 1 |
| EK1330-ST | Lyophilized recombinant mouse Chemerin standard | 50ng/tube×2 |
| EK1330-DA | Biotinylated anti- mouse Chemerin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1599 | 7530 | 22585 | 1551 | 7093 | 23589 |
| Standard deviation | 100.73 | 444.27 | 1558.36 | 97.71 | 524.88 | 1957.88 |
| CV(%) | 6.3% | 5.9% | 6.9% | 6.3% | 7.4% | 8.3% |
典型数据示例
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.042 | 0.109 | 0.17 | 0.305 | 0.558 | 0.915 | 1.749 | 2.004 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)