产品名称
Human Chemerin/RARRES2 Quick ELISA Kit
指标别名
Chemerin; HP10433; RAR responsive protein TIG2; RARRES2; TIG2
免疫原
Expression system for standard: E.coli; Immunogen sequence: V17-S163
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
retinoic acid receptor responder (tazarotene induced) 2
文献引用格式
Human Chemerin/RARRES2 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1329)
背景资料
Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
试剂盒内容
| Catalog number | Description | Quantity |
| FEK0663-CAP | 96-well plate precoated with anti-Human RARRES2 antibody | 1 |
| FEK0663-ST | lyophilized recombinant Human RARRES2 standard | 10ng/tube×2 |
| FEK0663-A | Human RARRES2 Antibody A | 3ml |
| FEK1329-B | Human RARRES2 Antibody B | 3ml |
| AR1106-1 | Sample Diluent | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 118 | 754 | 3266 | 128 | 779 | 3354 |
| Standard deviation | 6.49 | 47.5 | 192.69 | 6.66 | 57.65 | 197.89 |
| CV(%) | 5.5% | 6.3% | 5.9% | 5.2% | 7.4% | 5.9% |
典型数据示例
| Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 |
| O.D. | 0.013 | 0.119 | 0.195 | 0.356 | 0.623 | 1.012 | 1.389 | 1.802 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)