产品名称
Human CES1 ELISA Kit
指标别名
ACAT; Brain carboxylesterase hBr1; CE1; CEH; CES1; Cocaine carboxylesterase; Egasyn; HMSE; HMSE1; Liver carboxylesterase 1; PCE 1; REH; Retinyl ester hydrolase; Serine esterase 1; SES1; TGH; Triacylglycerol hydrolase
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
0.78 ng/ml - 50 ng/ml
文献引用格式
Human CES1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1813)
背景资料
Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. This gene is mapped to 16q12.2. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1813-CAP | 96-well plate precoated with anti- human CES1 antibody | 1 |
| EK1813-ST | Lyophilized recombinant human CES1 standard | 50ng/tube×2 |
| EK1813-DA | Biotinylated anti- human CES1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1486 | 5674 | 24323 | 1499 | 6001 | 24678 |
| Standard deviation | 62.41 | 249.66 | 1289.12 | 97.44 | 306.05 | 1801.49 |
| CV(%) | 4.2 | 4.4 | 5.3 | 6.5 | 5.1 | 7.3 |
典型数据示例
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.044 | 0.1 | 0.164 | 0.327 | 0.557 | 0.959 | 1.776 | 2.49 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)