产品名称
Mouse JAM-C/JAM3 ELISA Kit
指标别名
Junctional adhesion molecule C; JAM-C; Junctional adhesion molecule 3; JAM-3; Jam3
免疫原
Expression system for standard: NS0; Immunogen sequence: E30-N241
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
junction adhesion molecule 3
文献引用格式
Mouse JAM-C/JAM3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1840)
背景资料
JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 9; 9 A4. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts.
试剂盒内容
Catalog number | Description | Quantity |
EK1840-CAP | 96-well plate precoated with anti- mouse JAM-C/JAM3 antibody | 1 |
EK1840-ST | Lyophilized recombinant mouse JAM-C/JAM3 standard | 10ng/tube×2 |
EK1840-DA | Biotinylated anti- mouse JAM-C/JAM3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 58 | 270 | 884 | 61 | 279 | 991 |
Standard deviation | 2.90 | 14.04 | 39.78 | 2.81 | 17.86 | 74.33 |
CV(%) | 5.0% | 5.2% | 4.5% | 4.6% | 6.4% | 7.5% |
典型数据示例
Concentration(pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.007 | 0.070 | 0.183 | 0.314 | 0.490 | 0.896 | 1.512 | 2.129 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)