产品名称
Mouse JAM-C/JAM3 ELISA Kit
指标别名
Junctional adhesion molecule C; JAM-C; JAM-2; Junctional adhesion molecule 3; JAM-3; Jam3; JAM3
免疫原
Expression system for standard: NS0; Immunogen sequence: V32-N241
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
junctional adhesion molecule 3
文献引用格式
Mouse JAM-C/JAM3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1844)
背景资料
This gene is mapped to 11q25. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1844-CAP | 96-well plate precoated with anti- human JAM-C antibody | 1 |
EK1844-ST | Lyophilized recombinant human JAM-C standard | 10ng/tube×2 |
EK1844-DA | Biotinylated anti- human JAM-C antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 90 | 422 | 1408 | 92 | 430 | 1466 |
Standard deviation | 4.86 | 23.63 | 57.73 | 4.32 | 27.09 | 104.09 |
CV(%) | 5.4% | 5.6% | 4.1% | 4.7% | 6.3% | 7.1% |
典型数据示例
Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.026 | 0.153 | 0.204 | 0.429 | 0.767 | 0.389 | 1.692 | 2.226 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)