产品名称
Human/Mouse COX2/Cyclooxygenase 2/PTGS2 ELISA Kit
指标别名
COX 2; COX2; COX2/ Cyclooxygenase 2; COX2/ Cyclooxygenase 2/ PTGS2; Cyclooxygenase 2; GRIPGHS; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PHS 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; PTGS2
免疫原
Expression system for standard: NS0; Immunogen sequence: A18-L604
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10,000 pg/ml
基因名全称
prostaglandin-endoperoxide synthase 2
文献引用格式
Human/Mouse COX2/Cyclooxygenase 2/PTGS2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2289)
背景资料
Cyclooxygenase-2 (COX-2), also known as Prostaglandin-endoperoxide synthase 2 (HUGO PTGS2), is an enzyme that in humans is encoded by the PTGS2 gene. PTGS2 (prostaglandin-endoperoxide synthase 2) is a gene that encodes an enzyme responsible for catalyzing the conversion of arachidonic acid into prostaglandins. Prostaglandins are lipid signaling molecules that play crucial roles in regulating inflammation, pain, and reproductive processes. PTGS2 is expressed in various tissues, particularly in sites of inflammation and certain tumor cells, where its expression levels are often significantly elevated. The activity of this gene is regulated by various stimuli, including cytokines, hormones, and growth factors, making it a key player in pathological conditions such as inflammation, cancer, and cardiovascular diseases. Due to its involvement in these diseases, PTGS2 has become a potential drug target, especially in the development of nonsteroidal anti-inflammatory drugs (NSAIDs).
试剂盒内容
| Catalog number | Description | Quantity |
| EK2289-CAP | 96-well plate precoated with anti- Human/Mouse COX2/Cyclooxygenase 2/PTGS2 ELISA Kit A antibody | 1 |
| EK2289-ST | Lyophilized recombinant Human/Mouse COX2/Cyclooxygenase 2/PTGS2 ELISA Kit A standard | 10ng/tube×2 |
| EK2289 -DA | Biotinylated anti- Human/Mouse COX2/Cyclooxygenase 2/PTGS2 ELISA Kit A antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 272 | 936 | 2278 | 187/td> | 639 | 4978 |
| Standard deviation | 9.36 | 41.56 | 185.01 | 4.64 | 45.50 | 173.73 |
| CV(%) | 3.4% | 4.4% | 6.7% | 2.5% | 7.1% | 3.5% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.053 | 0.13 | 0.203 | 0.331 | 0.591 | 1.08 | 1.738 | 2.604 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
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