Anti-CD44 Antibody (Clone#OTI1E3)

Mouse monoclonal antibody

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M00052-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD44 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
all(8)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
all(8)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
all(8)
Anti-CD44 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD44 .
all(8)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
all(8)
Flow cytometric Analysis of living Colo205 cells, using anti-CD44 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD44 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD44.
all(8)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CD44 Antibody (Clone#OTI1E3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD44; CD44 antigen; CDW44; CSPG8; ECMR III; Epican; HCELL; Heparan sulfate proteoglycan; Hermes antigen; HUTCH I; Hyaluronate receptor; IN; MC56; MDU2; MDU3; MIC4; MUTCH I; PGP 1; PGP I; Pgp1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

CD44

克隆号

OTI1E3

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human CD44(NP_000601) produced in HEK293T cell.

计算分子量

82 kDa

实际分子量

82 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The CD44 gene, which is a transmembrane protein, is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. The gene, which contains 19 exons spanning some 50 kb of genomic DNA, is a widely expressed integral membrane protein that acts as a receptor for hyaluronan(HA) and is important to cell-extracellular matrix interaction. CD44 binding with HA can play an important role in cellular aggregation and tumor cell growth. CD44 is necessary for limb development and functions in a novel growth factor presentation mechanism. A specific CD44 splice variant is crucial for the proliferation of these mesenchymal cells. CD44 glycoproteins are involved in leukocyte extravasation but also in the regulation of growth factor activation, stability, and signaling. Moreover, it plays a pivotal role in arteriogenesis.

Uniprot ID

P16070

文献引用格式

CD44 Antibody (Clone#OTI1E3) (Boster Biological Technology, Wuhan, China. Catalog#M00052-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD44 molecule(Indian blood group)

蛋白名全称

CD44 antigen

实验方案>

实验步骤

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