Anti-FOXO1 Antibody (Clone#18F20)

Rabbit monoclonal antibody

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筛选器： All WB IHC

M00073-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-FOXO1 antibody (M00073-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOXO1 antigen affinity purified monoclonal antibody (M00073-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FOXO1 at approximately 70 kDa. The expected band size for FOXO1 is at 70 kDa.
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产品名称

Anti-FOXO1 Antibody (Clone#18F20)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

FKH1; FKHR; forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO1; FOXO1A

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

FOXO1

克隆号

18F20

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human FOXO1

计算分子量

70 kDa

实际分子量

70 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Forkhead box protein O1, also known as FKHR or FOXO1A, is a protein that in humans is encoded by the FOXO1 gene. It is mapped to 13q14.11. This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. FOXO1 plays important roles in regulation of gluconeogenesis and glycogenolysis by insulin signaling, and is also central to the decision for a preadipocyte to commit to adipogenesis. This gene is primarily regulated through phosphorylation on multiple residues. Its transcriptional activity is dependent on its phosphorylation state. What's more, FOXO1 may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Uniprot ID

Q12778

文献引用格式

FOXO1 Antibody (Clone#18F20) (Boster Biological Technology, Wuhan, China. Catalog#M00073-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Forkhead box protein O1|Forkhead box protein O1A|Forkhead in rhabdomyosarcoma

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实验步骤

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