Anti-BTK Antibody (Clone#OTI12D4)

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of anti-BTK antibody (M00245-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HEL whole cell lysates,
  Lane 2: human REH whole cell lysates,
  Lane 3: human Daudi whole cell lysates,
  Lane 4: human Ramos whole cell lysates,
  Lane 5: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BTK antigen affinity purified monoclonal antibody (M00245-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BTK at approximately 76 kDa. The expected band size for BTK is at 76 kDa.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00245-3, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00245-3, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00245-3, Dilution 1:50)

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  Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK .

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  HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal (M00245-3), and then analyzed by flow cytometry.

• 

  Western blot analysis of anti-BTK antibody (M00245-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HEL whole cell lysates,
  Lane 2: human REH whole cell lysates,
  Lane 3: human Daudi whole cell lysates,
  Lane 4: human Ramos whole cell lysates,
  Lane 5: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BTK antigen affinity purified monoclonal antibody (M00245-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BTK at approximately 76 kDa. The expected band size for BTK is at 76 kDa.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00245-3, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00245-3, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00245-3, Dilution 1:50)

• 

  Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK .

• 

  HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal (M00245-3), and then analyzed by flow cytometry.