Western blot analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BSAP/PAX5 antigen affinity purified monoclonal antibody (M00669-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSAP/PAX5 at approximately 45 kDa. The expected band size for BSAP/PAX5 is at 42 kDa.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of PAX5 using anti- PAX5 antibody (M00669-2)
PAX5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti- PAX5 Antibody (M00669-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BSAP/PAX5 antigen affinity purified monoclonal antibody (M00669-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSAP/PAX5 at approximately 45 kDa. The expected band size for BSAP/PAX5 is at 42 kDa.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of PAX5 using anti- PAX5 antibody (M00669-2)
PAX5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti- PAX5 Antibody (M00669-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BSAP/PAX5 antigen affinity purified monoclonal antibody (M00669-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSAP/PAX5 at approximately 45 kDa. The expected band size for BSAP/PAX5 is at 42 kDa.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of PAX5 using anti- PAX5 antibody (M00669-2)
PAX5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti- PAX5 Antibody (M00669-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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