Anti-Alpha 1 Antitrypsin/SERPINA1 Antibody (Clone#OTI9A1)

Mouse monoclonal antibody

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筛选器： All WB IHC FCM

M00720-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.
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Western blot analysis of extracts (10ug) from 7 Human tissue by using anti-SERPINA1 monoclonal antibody (1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1(Cat# M00720-3).
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00720-3)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00720-3)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00720-3)
all(9)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00720-3)
all(9)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00720-3)
all(9)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINA1 antibody, and then analyzed by flow cytometry.
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产品简介>

产品名称

Anti-Alpha 1 Antitrypsin/SERPINA1 Antibody (Clone#OTI9A1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

A1A; A-1-Antitrypsin; A1AT; AAT; Alpha 1 antiproteinase; Alpha 1 antitrypsin; Alpha 1 protease inhibitor; PI; PI1; PRO2209; PRO2275; Serpin A1; SERPINA1; SPAAT; α1-antitrypsin

产品类型

Monoclonal

检验物种

human, dog, monkey

应用范围

WB, IHC, FCM

基因名称

SERPINA1

克隆号

OTI9A1

宿主

Mouse

抗体亚型

IgG1

免疫原

Full-length protein expressed in 293T cell transfected with human SERPINA1 expression vector

计算分子量

46 kDa

实际分子量

46.7 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase,plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

Uniprot ID

P17475

文献引用格式

Alpha 1 Antitrypsin/SERPINA1 Antibody (Clone#OTI9A1) (Boster Biological Technology, Wuhan, China. Catalog#M00720-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1

蛋白名全称

Alpha-1-antiproteinase

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