Anti-p130 Cas/BCAR1 Antibody (Clone#OTI3A11)

Mouse monoclonal antibody

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M00960-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00960-3)
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCAR1 antibody, and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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Anti-BCAR1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1 .
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Flow cytometric Analysis of Jurkat cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-p130 Cas/BCAR1 Antibody (Clone#OTI3A11)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

BCAR1; CAS; CAS1; CASS1; CRK associated substrate; CRKAS; P130Cas

产品类型

Monoclonal

检验物种

human, mouse, rat, dog, monkey

应用范围

WB, IHC, ICC/IF, FCM

基因名称

BCAR1

克隆号

OTI3A11

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human BCAR1 (NP_055382) produced in HEK293T cell.

计算分子量

93 kDa

实际分子量

93.2 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]). [supplied by OMIM]

Uniprot ID

P56945

文献引用格式

p130 Cas/BCAR1 Antibody (Clone#OTI3A11) (Boster Biological Technology, Wuhan, China. Catalog#M00960-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Breast cancer anti-estrogen resistance protein 1|CRK-associated substrate|Cas scaffolding protein family member 1|p130cas

实验方案>

实验步骤

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