Anti-CNPase/CNP Antibody (Clone#OTI3C3)

Mouse monoclonal antibody

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筛选器： All WB IHC

M01017-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNP (1:2000) (Cat# M01017-4).
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Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-CNP monoclonal antibody (1:500).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and CNP-Knockout hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-CNP monoclonal antibody M01017-4. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([MA01692]) as a loading control (1:500).
all(4)
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-CNP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01017-4) (1:500)
all(4)

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产品简介>

产品名称

Anti-CNPase/CNP Antibody (Clone#OTI3C3)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CNP; CNP1; CNPase

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

CNP

克隆号

OTI3C3

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombiant protein of human CNP (NP_149124) produced in HEK293T cell.

计算分子量

48 kDa

实际分子量

47.4 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

2',3'-Cyclic-nucleotide 3'-phosphodiesterase, also known as CNPase, is an enzyme that in humans is encoded by the CNP gene. And this gene is mapped to 17q21.2. CNPase is named for its ability to catalyze the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. CNPase is thought to play a critical role in the events leading up to myelination. Additionally, CNPase has been demonstrated to inhibit the replication of HIV-1 and other primate lentiviruses by binding the retroviral Gag protein and inhibiting the genesis of nascent viral particles.

Uniprot ID

P09543

文献引用格式

CNPase/CNP Antibody (Clone#OTI3C3) (Boster Biological Technology, Wuhan, China. Catalog#M01017-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

2',3'-cyclic nucleotide 3' phosphodiesterase

蛋白名全称

2',3'-cyclic-nucleotide 3'-phosphodiesterase

实验方案>

实验步骤

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