Anti-CNPase/CNP Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM ELISA

A01017-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CNPase/CNP antigen affinity purified polyclonal antibody (A01017-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CNPase/CNP at approximately 48 kDa. The expected band size for CNPase/CNP is at 48 kDa.
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IHC analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4).
CNPase/CNP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-CNPase/CNP Antibody (A01017-4) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4).
CNPase/CNP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CNPase/CNP Antibody (A01017-4) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4).
CNPase/CNP was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-CNPase/CNP Antibody (A01017-4) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis using anti- CNP antibody (A01017-4). detected in paraffin-embedded section of mouse brain tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135) and counterstained with DAPI (blue).
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IF analysis using anti- CNP antibody (A01017-4). detected in paraffin-embedded section of rat brain tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135) and counterstained with DAPI (blue).
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Flow Cytometry analysis of U937 cells using anti-CNPase/CNP antibody (A01017-4).
Overlay histogram showing U937 cells stained with A01017-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CNPase/CNP Antibody (A01017-4) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CNPase/CNP Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CNP; CNP1; CNPase

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, FCM, ELISA

基因名称

CNP

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CNPase/CNP recombinant protein (Position: M1-I421).

计算分子量

48 kDa

实际分子量

48 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

2',3'-Cyclic-nucleotide 3'-phosphodiesterase, also known as CNPase, is an enzyme that in humans is encoded by the CNP gene. And this gene is mapped to 17q21.2. CNPase is named for its ability to catalyze the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. CNPase is thought to play a critical role in the events leading up to myelination. Additionally, CNPase has been demonstrated to inhibit the replication of HIV-1 and other primate lentiviruses by binding the retroviral Gag protein and inhibiting the genesis of nascent viral particles.

Uniprot ID

P09543

文献引用格式

CNPase/CNP Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01017-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

2',3'-cyclic nucleotide 3' phosphodiesterase

蛋白名全称

2',3'-cyclic-nucleotide 3'-phosphodiesterase

实验方案>

实验步骤

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