Anti-Beta Actin/ACTB Antibody (Clone#17A10)

Mouse monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF

M01263-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-Beta Actin/ACTB antibody (M01263-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: pig brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Beta Actin/ACTB antigen affinity purified monoclonal antibody (M01263-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.
all(4)
Western blot analysis of anti-Beta Actin/ACTB antibody (M01263-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat NRK whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates,
Lane 6: chicken liver tissue lysates,
Lane 7: chicken brain tissue lysates,
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Beta Actin/ACTB antigen affinity purified monoclonal antibody (M01263-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.
all(4)
Immunohistochemical analysis of paraffin-embedded human heart, using beta Actin Mouse Monoclonal Antibody.
all(4)
Immunofluorescent analysis of HepG2 cells, using beta Actin mAb.
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Beta Actin/ACTB Antibody (Clone#17A10)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ACTB; Beta-actin; B actin; Beta actin; PS1TP5BP1; β actin; BRWS1

产品类型

Monoclonal

检验物种

human, mouse, rat, chicken, rabbit

应用范围

WB, IHC, ICC/IF

基因名称

ACTB

克隆号

17A10

宿主

Mouse

抗体亚型

IgG1

免疫原

A synthesized peptide derived from human beta Actin

计算分子量

42 kDa

实际分子量

42 kDa(不适用于心肌样本的检测)

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Uniprot ID

P60709

文献引用格式

Beta Actin/ACTB Antibody (Clone#17A10) (Boster Biological Technology, Wuhan, China. Catalog#M01263-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Actin, cytoplasmic 1

实验方案>

实验步骤

引用文献(0)>

清除