Anti-ALDH1A1 Antibody (Clone#4C3)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF

M01392-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ALDH1A1 using anti-ALDH1A1 antibody (M01392-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ALDH1A1 antigen affinity purified monoclonal antibody (M01392-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALDH1A1 at approximately 55 kDa. The expected band size for ALDH1A1 is at 55 kDa.
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IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (M01392-2).
ALDH1A1 was detected in a paraffin-embedded section of human Gall bladder adenosquamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ALDH1A1 Antibody (M01392-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (M01392-2).
ALDH1A1 was detected in a paraffin-embedded section of human lymphadenoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ALDH1A1 Antibody (M01392-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IF analysis of ALDH1A1 using anti-ALDH1A1 antibody (M01392-2).
ALDH1A1 was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-ALDH1A1 Antibody (M01392-2) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(4)

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产品名称

Anti-ALDH1A1 Antibody (Clone#4C3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ALDC; ALDH E1; ALDH1; ALDH11; ALDH1A1; ALHDII; PUMB1; RALDH 1; RALDH1; Retinal dehydrogenase 1

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF

基因名称

ALDH1A1

克隆号

4C3

宿主

Mouse

抗体亚型

IgG2a

免疫原

E.coli-derived human ALDH1A1 recombinant protein (Position: T6-Q342).

计算分子量

55 kDa

实际分子量

55 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Aldehyde dehydrogenase 1 family, member A1, also known as ALDH1A1 or retinaldehyde dehydrogenase 1 (RALDH1), is an enzyme that in humans is encoded by the ALDH1A1 gene. It is mapped to 9q21.13. The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.

Uniprot ID

P00352

文献引用格式

ALDH1A1 Antibody (Clone#4C3) (Boster Biological Technology, Wuhan, China. Catalog#M01392-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

aldehyde dehydrogenase 1 family member A1

蛋白名全称

Retinal dehydrogenase 1

实验方案>

实验步骤

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