Anti-ERp57/ERp60/PDIA3 Antibody (Clone#OTI3D2)

Mouse monoclonal antibody

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筛选器： All WB

M01464-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDIA3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
all(2)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA3.
all(2)

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产品名称

Anti-ERp57/ERp60/PDIA3 Antibody (Clone#OTI3D2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

58 kDa microsomal protein; Disulfide isomerase ER 60; endoplasmic reticulum marker; ER protein 57; ER protein 60; ER60; ERp57; ERp57/ERp60; ERp60; ERp61; GRP57; GRP58; HsT17083; P58; PDIA3; PI PLC; Protein disulfide isomerase A3

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

PDIA3

克隆号

OTI3D2

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 140-505 of human PDIA3(NP_005304) produced in E.coli.

计算分子量

57 kDa

实际分子量

54.2 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

Uniprot ID

P30101

文献引用格式

ERp57/ERp60/PDIA3 Antibody (Clone#OTI3D2) (Boster Biological Technology, Wuhan, China. Catalog#M01464-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

protein disulfide isomerase family A, member 3

蛋白名全称

Protein disulfide-isomerase A3

实验方案>

实验步骤

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