Anti-IQGAP1 Antibody (Clone#OTI6E10)

Mouse monoclonal antibody

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筛选器： All IHC WB

M01603-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IQGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01603-2) (1:2000)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IQGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01603-2) (1:2000)
all(4)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IQGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IQGAP1 (1:2000).
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Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-IQGAP1 monoclonal antibody (1:500).
all(4)

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产品简介>

产品名称

Anti-IQGAP1 Antibody (Clone#OTI6E10)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

HUMORFA01; IQGAP1; KIAA0051; p195; SAR1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

IQGAP1

克隆号

OTI6E10

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 464-609 of human IQGAP1(NP_003861) produced in E.coli.

实际分子量

189.1 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]

Uniprot ID

P46940

文献引用格式

IQGAP1 Antibody (Clone#OTI6E10) (Boster Biological Technology, Wuhan, China. Catalog#M01603-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

IQ motif containing GTPase activating protein 1

蛋白名全称

Ras GTPase-activating-like protein IQGAP1

实验方案>

实验步骤

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