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Anti-DSG2 Antibody (Clone#2B4D1)

Mouse monoclonal antibody

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筛选器: All WB IHC

M02035-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of DSG2 using anti-DSG2 antibody (M02035-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human K562 whole cell lysates,
    Lane 3: human HepG2 whole cell lysates,
    Lane 4: human A549 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-DSG2 antigen affinity purified monoclonal antibody (M02035-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DSG2 at approximately 160 kDa. The expected band size for DSG2 is at 122 kDa.

    all(5)
  • IHC analysis of DSG2 using anti-DSG2 antibody (M02035-2).
    DSG2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with mouse anti-DSG2 Antibody (M02035-2) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of DSG2 using anti-DSG2 antibody (M02035-2).
    DSG2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with mouse anti-DSG2 Antibody (M02035-2) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of DSG2 using anti-DSG2 antibody (M02035-2).
    DSG2 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. The tissue section was incubated with mouse anti-DSG2 Antibody (M02035-2) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of DSG2 using anti-DSG2 antibody (M02035-2).
    DSG2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. The tissue section was incubated with mouse anti-DSG2 Antibody (M02035-2) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

    all(5)

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产品名称
Anti-DSG2 Antibody (Clone#2B4D1)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
ARVC10; ARVD10; Cadherin family member 5; CDHF5; desmoglein 2; DSG2; HDGC
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC
基因名称
DSG2
克隆号
2B4D1
宿主
Mouse
抗体亚型
IgG1
免疫原
E.coli-derived human Desmoglein 2/DSG2 recombinant protein (Position: L24-E1020).
计算分子量
122 kDa
实际分子量
160 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in DSG2 display a high degree of penetrance. Disease expression was of variable severity with LV involvement a prominent feature. The low prevalence of classical ECG changes highlights the need to expand current diagnostic criteria to take account of LV disease, childhood disease expression, and incomplete penetrance.
Uniprot ID
Q14126  
文献引用格式
DSG2 Antibody (Clone#2B4D1) (Boster Biological Technology, Wuhan, China. Catalog#M02035-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
基因名全称
desmoglein 2
蛋白名全称
Desmoglein-2
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-DSG2 Antibody (Clone#2B4D1)

    筛选器: All WB IHC

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