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Anti-HIF-1 Beta/ARNT Antibody (Clone#OTI1A1)

Mouse monoclonal antibody

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筛选器: All WB IHC ICC/IF FCM

M02263-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Western blot analysis of anti-HIF-1 Beta/ARNT antibody (M02263-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human 293T whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human HepG2 whole cell lysates,
    Lane 4: human THP-1 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-HIF-1 Beta/ARNT antigen affinity purified monoclonal antibody (M02263-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HIF-1 Beta/ARNT at approximately 87 kDa. The expected band size for HIF-1 Beta/ARNT is at 87 kDa.

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  • Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02263-2)

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02263-2)

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02263-2)

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02263-2)

    all(14)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02263-2)

    all(14)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02263-2)

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02263-2)

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02263-2)

    all(14)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02263-2)

    all(14)
  • Anti-ARNT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNT .

    all(14)
  • Figure from citation: Immunofluorescence of ARNT protein level by using anti-ARNT antibody in mouse neuronal cells. View Citation

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  • HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARNT antibody, and then analyzed by flow cytometry.

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  • Flow cytometric Analysis of Jurkat cells, using anti-ARNT antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

    all(14)

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产品简介>

产品名称
Anti-HIF-1 Beta/ARNT Antibody (Clone#OTI1A1)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
ARNT; ARNT protein; bHLHe2; HIF 1 beta; HIF 1beta; HIF1 beta; HIF1B; HIF1BETA; TANGO
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
ARNT
克隆号
OTI1A1
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human ARNT (NP_001659) produced in HEK293T cell.
计算分子量
87 kDa
实际分子量
87 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene.
Uniprot ID
P27540  
文献引用格式
HIF-1 Beta/ARNT Antibody (Clone#OTI1A1) (Boster Biological Technology, Wuhan, China. Catalog#M02263-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
aryl hydrocarbon receptor nuclear translocator
蛋白名全称
Aryl hydrocarbon receptor nuclear translocator
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:50
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100
Flow cytometry (FCM):1:100

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    Anti-HIF-1 Beta/ARNT Antibody (Clone#OTI1A1)

    筛选器: All WB IHC ICC/IF FCM

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