Anti-HIF-1 Beta/ARNT Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0147

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HIF-1 Beta/ARNT using anti-HIF-1 Beta/ARNT antibody (PB0147).
Lane 1: recombinant Human HIF1 beta Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HIF-1 Beta/ARNT antigen affinity purified polyclonal antibody (PB0147) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HIF-1 Beta/ARNT at approximately 49 kDa.
all(2)
Western blot analysis of HIF-1 Beta/ARNT using anti-HIF-1 Beta/ARNT antibody (PB0147). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: Jurkat whole cell lysates,
Lane 4: U87 whole cell lysates,
Lane 5: Colo320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HIF-1 Beta/ARNT antigen affinity purified polyclonal antibody (PB0147) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HIF-1 Beta/ARNT at approximately 87 kDa. The expected band size for HIF-1 Beta/ARNT is at 87 kDa.
all(2)

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产品简介>

产品名称

Anti-HIF-1 Beta/ARNT Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ARNT; ARNT protein; bHLHe2; HIF 1 beta; HIF 1beta; HIF1 beta; HIF1B; HIF1BETA; TANGO

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

ARNT

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human HIF1 beta recombinant protein (Position: V416-E789). Human HIF1 beta shares 86% and 83% amino acid (aa) sequences identity with mouse and rat HIF1 beta, respectively.

计算分子量

87 kDa

实际分子量

87 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ARNT is also known as HIF1-beta or HIF1B. This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. It is mapped to 1q21.3. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. ARNT is a structural component of the XRE-binding form of the Ah receptor. It also functions in concert with RelB in a CD30-induced negative feedback mechanism.

Uniprot ID

P27540

文献引用格式

HIF-1 Beta/ARNT Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0147)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

aryl hydrocarbon receptor nuclear translocator

蛋白名全称

Aryl hydrocarbon receptor nuclear translocator

实验方案>

实验步骤

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