Anti-GSDMD Antibody (Clone#3D5)

Mouse monoclonal antibody

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筛选器： All WB IHC

M02842-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of GSDMD using anti-GSDMD antibody (M02842-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: THP-1 whole cell lysates,
Lane 2: SW620 whole cell lysates,
Lane 3: Jurkat whole cell lysates,
Lane 4: U87 whole cell lysates,
Lane 5: PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GSDMD antigen affinity purified monoclonal antibody (M02842-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GSDMD at approximately 53,30 kDa. The expected band size for GSDMD is at 53 kDa.
all(4)
IHC analysis of GSDMD using anti-GSDMD antibody (M02842-1).
GSDMD was detected in a paraffin-embedded section of human esophagus tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GSDMD Antibody (M02842-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of GSDMD using anti-GSDMD antibody (M02842-1).
GSDMD was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GSDMD Antibody (M02842-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of GSDMD using anti-GSDMD antibody (M02842-1).
GSDMD was detected in a paraffin-embedded section of human lymph gland tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GSDMD Antibody (M02842-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-GSDMD Antibody (Clone#3D5)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

DF5L; DFNA5L; FKSG10; gasdermin D; GSDMD; GSDMDC1

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC

基因名称

GSDMD

克隆号

3D5

宿主

Mouse

抗体亚型

IgG2a

免疫原

E.coli-derived human GSDMD recombinant protein (Position: M1-H484).

计算分子量

53 kDa

实际分子量

53,30 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Uniprot ID

P57764

RRID

AB_3082481

文献引用格式

GSDMD Antibody (Clone#3D5) (Boster Biological Technology, Wuhan, China. Catalog#M02842-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

gasdermin D

蛋白名全称

Gasdermin-D

实验方案>

实验步骤

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