Anti-NR1I3 Antibody (Clone#OTI1B2)

Mouse monoclonal antibody

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筛选器： All IHC WB

M02858-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NR1I3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M02858-1)
all(3)
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-NR1I3 monoclonal antibody (1:200).
all(3)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1I3.
all(3)

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产品简介>

产品名称

Anti-NR1I3 Antibody (Clone#OTI1B2)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CAR; CAR BETA; CAR1; Constitutive active response; MB67; NR1I3; Orphan nuclear receptor MB67

产品类型

Monoclonal

检验物种

human

应用范围

IHC, WB

基因名称

NR1I3

克隆号

OTI1B2

宿主

Mouse

抗体亚型

IgG2a

免疫原

Human recombinant protein fragment corresponding to amino acids 1-236 of human NR1I3 (NP_001070950) produced in E.coli.

计算分子量

40 kDa

实际分子量

40.3 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot ID

Q14994

文献引用格式

NR1I3 Antibody (Clone#OTI1B2) (Boster Biological Technology, Wuhan, China. Catalog#M02858-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Nuclear receptor subfamily 1 group I member 3

实验方案>

实验步骤

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