Anti-Aconitase 2/ACO2 Antibody (Clone#4C12D1)

Mouse monoclonal antibody

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筛选器： All WB IHC

M03096-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: Jurkat whole cell lysates,
Lane 4: SH-SY5Y whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Aconitase 2/ACO2 antigen affinity purified monoclonal antibody (M03096-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Aconitase 2/ACO2 at approximately 85 kDa. The expected band size for Aconitase 2/ACO2 is at 85 kDa.
all(8)
IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
Aconitase 2/ACO2 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
Aconitase 2/ACO2 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
Aconitase 2/ACO2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
Aconitase 2/ACO2 was detected in a paraffin-embedded section of human Lung adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
Aconitase 2/ACO2 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
Aconitase 2/ACO2 was detected in a paraffin-embedded section of rat heart tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
Aconitase 2/ACO2 was detected in a paraffin-embedded section of mouse heart tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)

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产品简介>

产品名称

Anti-Aconitase 2/ACO2 Antibody (Clone#4C12D1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ACO2; Aconitase; aconitase 2; aconitase 2; mitochondrial; ACONM; Citrate hydro lyase

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

ACO2

克隆号

4C12D1

宿主

Mouse

抗体亚型

IgG1

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Aconitase 2, identical to the related mouse and rat sequences.

计算分子量

85 kDa

实际分子量

85 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Aconitase 2, mitochondrial is a protein that in humans is encoded by the ACO2 gene. The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

Uniprot ID

Q99798

文献引用格式

Aconitase 2/ACO2 Antibody (Clone#4C12D1) (Boster Biological Technology, Wuhan, China. Catalog#M03096-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

aconitase 2

蛋白名全称

Aconitate hydratase, mitochondrial

实验方案>

实验步骤

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