Anti-Alpha Tubulin/TUBA1A Antibody (Clone#7B12)

Mouse monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF FCM

M03989-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (M03989-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Alpha Tubulin/TUBA1A antigen affinity purified monoclonal antibody (M03989-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha Tubulin/TUBA1A at approximately 55 kDa. The expected band size for Alpha Tubulin/TUBA1A is at 50 kDa.
all(8)
IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (M03989-3).
Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alpha Tubulin/TUBA1A Antibody (M03989-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (M03989-3).
Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alpha Tubulin/TUBA1A Antibody (M03989-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (M03989-3).
Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alpha Tubulin/TUBA1A Antibody (M03989-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (M03989-3).
Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alpha Tubulin/TUBA1A Antibody (M03989-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (M03989-3).
Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alpha Tubulin/TUBA1A Antibody (M03989-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IF analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (M03989-3).
Alpha Tubulin/TUBA1A was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-Alpha Tubulin/TUBA1A Antibody (M03989-3) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(8)
Flow Cytometry analysis of A431 cells using anti-Alpha Tubulin/TUBA1A antibody (M03989-3).
Overlay histogram showing A431 cells stained with M03989-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Alpha Tubulin/TUBA1A Antibody (M03989-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(8)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Alpha Tubulin/TUBA1A Antibody (Clone#7B12)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

alpha tubulin; TUBA1A; TUBA3

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

TUBA1A

克隆号

7B12

宿主

Mouse

抗体亚型

IgG2b

免疫原

E.coli-derived human Tubulin alpha recombinant protein (Position: N18-A403).

计算分子量

50 kDa

实际分子量

55 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Uniprot ID

Q71U36

文献引用格式

Alpha Tubulin/TUBA1A Antibody (Clone#7B12) (Boster Biological Technology, Wuhan, China. Catalog#M03989-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tubulin alpha 1a/tubulin alpha 1b/tubulin alpha 1c

蛋白名全称

Tubulin alpha-1A chain/Tubulin alpha-1B chain/Tubulin alpha-1C chain

实验方案>

实验步骤

引用文献(0)>

清除