Anti-Alpha Tubulin/TUBA1A Antibody (Clone#OTI2C8)

Mouse monoclonal antibody

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筛选器： All IHC WB

M03989-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TUBA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M03989-4)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TUBA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M03989-4)
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBA1A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
all(7)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TUBA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M03989-4)
all(7)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TUBA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M03989-4)
all(7)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TUBA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M03989-4)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA1A(Cat# M03989-4).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Alpha Tubulin/TUBA1A Antibody (Clone#OTI2C8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

alpha tubulin; TUBA1A; TUBA3

产品类型

Monoclonal

检验物种

human, mouse, rat, dog, monkey

应用范围

IHC, WB

基因名称

TUBA1A

克隆号

OTI2C8

宿主

Mouse

抗体亚型

IgG2b

免疫原

Full length human recombinant protein of human TUBA1A(NP_006000) produced in HEK293T cell

计算分子量

50 kDa

实际分子量

50 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Uniprot ID

Q71U36

文献引用格式

Alpha Tubulin/TUBA1A Antibody (Clone#OTI2C8) (Boster Biological Technology, Wuhan, China. Catalog#M03989-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tubulin alpha 1a/tubulin alpha 1b/tubulin alpha 1c

蛋白名全称

Tubulin alpha-1A chain/Tubulin alpha-1B chain/Tubulin alpha-1C chain

实验方案>

实验步骤

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