Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Flow Cytometry (Fixed): | 1:50-200 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACBD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACBD3.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-ACBD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
HEK293T cells transfected with either ACBD3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACBD3 antibody (M05645)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACBD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACBD3.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-ACBD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
HEK293T cells transfected with either ACBD3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACBD3 antibody (M05645)