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Anti-ACBD3 Antibody (Clone#OTI1G2)

Mouse monoclonal antibody

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筛选器: All WB IHC FCM

M05645

  • 100μl ¥6276
  • 货期: 3-4周
  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACBD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACBD3.

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  • Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-ACBD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

    all(14)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  • Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

    all(14)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

    all(14)
  • Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  • HEK293T cells transfected with either ACBD3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACBD3 antibody (M05645)

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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-ACBD3 Antibody (Clone#OTI1G2)
规格/价格
100μl/6276
指标别称
ACBD3; GCP60; GOCAP1; Golgi phosphoprotein 1; Golgi resident protein GCP60; GOLPH1; PAP7
产品类型
Monoclonal
检验物种
human, mouse, rat, dog, monkey
应用范围
WB, IHC, FCM
基因名称
ACBD3
克隆号
OTI1G2
宿主
Mouse
免疫原
Full length human recombinant protein of human ACBD3 (NP_073572) produced in HEK293T cell.
计算分子量
61 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
1 mg/ml
保存条件
Store at -20°C as received.
Uniprot ID
Q9H3P7  
文献引用格式
ACBD3 Antibody (Clone#OTI1G2) (Boster Biological Technology, Wuhan, China. Catalog#M05645)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Flow Cytometry (Fixed):1:50-200
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-ACBD3 Antibody (Clone#OTI1G2)

    筛选器: All WB IHC FCM

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